Tissue Culture Master Class: Cannabis

Join Cannabis Tissue Culture Expert Bill Graham and the Plant Cell Technology Team For This 3-Day Hands-On Master Class From April 20 - 22, 2023! 

FOR GROWERS, DISPENSARY OWNERS, ENTHUSIASTS, AND MUCH MORE!

THE CANNABIS INDUSTRY IS GROWING FAST, AND TISSUE CULTURE IS YOUR SOLUTION TO KEEP UP WITH THE HIGH DEMAND. DON’T GET LEFT BEHIND; LEARN HOW YOU CAN START TISSUE CULTURING TODAY!

The event is a 3-day hands-on masterclass from April 20 - 22, 2023, led by THE Pioneer and Plant Tissue Culture Expert Bill Graham, and the Plant Cell Technology team. The event is open to growers, dispensary owners, enthusiasts, and anyone interested in learning about tissue culture in the cannabis industry.

Tissue culture media preparation is an essential aspect of plant development in tissue culture, and the seminar provides instructions, ingredients, and a list of materials required to prepare the media. Participants will learn about macro and micronutrients, vitamins, organic compounds, pH levels, pressure cookers, laminar flood hoods, and how to fill culture vessels. They will also learn about plant hormones and their importance in tissue culture.

The masterclass covers cannabis micropropagation, including induction, sterilization techniques, plant cutting, and explant preparation. Participants will learn about the different equipment and tools used in tissue culture and how to use them effectively. Additionally, the seminar covers pathogen remediation using meristem and gender screening using PCR.

Furthermore, the masterclass offers information on cutting-edge technology gender screening and long-term storage solutions. Participants will learn how to use synthetic seeds to store cannabis for a longer time. The event aims to equip attendees with the skills and knowledge needed to start a cannabis tissue culture business successfully.

Accurate media preparation is necessary to promote the development of plants in tissue culture. And, in the lack of accessible information on suitable media for Cannabis tissue culture, we are going to give you access to this and more in this seminar, including:

• Instructions, Ingredients, & List of Materials: Learn what macro and micronutrients, vitamins, and organic compounds are required and in what concentrations to prepare the media.
• Get Started With pH Levels: Learn when and how to adjust the pH of the tissue culture media.
• Intro to Pressure Cooker for Tissue Culture: Expand your knowledge of different types of pressure cookers and their uses in labs. And how to decide which one’s the best suited for your work.
• Intro to Laminar Flood Hood: Have you heard about this machine but are unsure what it is and why it’s important for tissue culture? Get all the answers related to the equipment in this masterclass.
• TC Hormones 101: Do not know how plant hormones work and how they can be used in tissue culture? No worries, because here we will cover what they are, their types, natural and synthetic hormones, and how they are essential to tissue culture.
• Intro to Vessel Filling: Learn the best practices of dispensing media in culture vessels and what amount will be ideally based on their sizes.

Overall, this section of the master class will provide students with a solid foundation of knowledge on the principles of tissue culture and the skills to prepare tissue culture media for cannabis. Students will learn how to make their own media with the appropriate nutrients and hormones necessary for successful plant growth in tissue culture.

During the Plant Selection, Leaf and Node Sterilization section of the cannabis tissue culture master class, students will learn how to select and prepare the plant material for tissue culture. This section will cover the selection of the best plants for tissue culture and the techniques for cleaning and sterilizing plant leaves and nodes before introducing them into tissue culture.

• Plant Selection:
  • Learn how to select the best plants for tissue culture.
  • Understand the importance of selecting disease-free plants and those with desirable traits.
  • Learn how to collect plant material such as leaves and nodes without damaging them.
• Leaf and Node Sterilization:
  • Learn about the different sterilization techniques used in tissue culture, such as using bleach, ethanol, or other chemicals.
  • Understand the importance of using sterile conditions and equipment during the sterilization process to avoid contamination.
  • Learn how to clean and sterilize plant leaves and nodes before introducing them into tissue culture.

Overall, this section of the master class will provide students with the knowledge and skills to select and prepare the best plant material for tissue culture while ensuring a sterile environment to avoid contamination.

During the Cannabis Micropropagation part of the master class, the students will learn how to propagate cannabis plants using tissue culture techniques. The topics covered will include:

• Induction: The experts will teach the students how to induce Cannabis growth in tissue culture. This involves the use of plant growth hormones and proper environmental conditions to promote plant development.
• Intro to Sterilization: Proper sterilization techniques are crucial for success in Cannabis micropropagation. Students will learn the appropriate methods for surface sterilization of plant material to eliminate contaminants.
• Plant Cuttings 101: Cutting plant material correctly is important to avoid damaging the explants. The experts will teach the students how to properly cut and handle plant material to obtain clean and viable explants for tissue culture.
• Explant Preparation (Clean & Contaminated Plants Techniques): Students will learn how to obtain clean and healthy plant tissues and prepare them for introduction into tissue culture, as well as how to handle contaminated plant material to prevent contamination in the culture.
• Bonus: Introduction to Tissue Culture Equipment: To start strong in their Cannabis tissue culture business, it is important to be familiar with the equipment and tools used. The experts will provide a bonus session to introduce the students to tissue culture equipment and their uses.

During this part of the cannabis tissue culture master class, the students will learn about the process of subculturing and resterilizing contaminated cultures in order to maintain healthy and viable plant cells for micropropagation. Specifically, the students will:

• Learn about the importance of subculturing and resterilizing in maintaining healthy plant cell cultures for micropropagation.
• Understand the steps involved in subculturing and resterilizing cultures, including the use of aseptic techniques to prevent contamination.
• Practice subculturing and resterilizing techniques on contaminated cultures under the guidance of the experts leading the master class.
• Learn how to identify and troubleshoot common issues that can arise during subculturing and resterilization, such as bacterial or fungal contamination.
• Develop a deeper understanding of the best practices for maintaining and managing plant cell cultures to ensure successful micropropagation.

Cannabis tissue culture is not easy. And when it comes to adding plant hormones, you must know what hormone, in what concentration, and how to add it to obtain the best results. Grab this secret from the industry experts in this only masterclass.

• Introduction to Meristem Dissection: Meristems are a type of plant tissue consisting of undifferentiated cells. Learn how they can be used for virus elimination in infected plants and obtain clean clones.
• Meristem Dissection Procedures: It needs some hands-on experience to cut meristem out of plants without damaging the tissues. Learn how exactly to do it from the experts in the industry in this masterclass.

During this part of the cannabis tissue culture master class, the students will learn about subculturing and resterilizing contaminated cultures to maintain healthy and viable plant cells for micropropagation. Specifically, the students will:

• Learn about the importance of subculturing and resterilizing in maintaining healthy plant cell cultures for micropropagation.
• Understand the steps involved in subculturing and resterilizing cultures, including the use of aseptic techniques to prevent contamination.
• Practice subculturing and resterilizing techniques on contaminated cultures under the guidance of the experts leading the master class.
• Learn how to identify and troubleshoot common issues that can arise during subculturing and resterilization, such as bacterial or fungal contamination.
• Develop a deeper understanding of the best practices for maintaining and managing plant cell cultures to ensure successful micropropagation.

Cutting-Edge Technology Gender-Screening at Young Age: Learn about all high-throughput technology available for gender screening and identify male and female plants in a fraction of the time at a young stage, rather than waiting for weeks to make the identification.

During this part of the cannabis tissue culture master class, students will learn how to use the Medicinal Genomics PCR machine for gender screening of their cannabis plants. The following is a breakdown of what the students will do:

• Learn the basics of PCR (polymerase chain reaction) and the design of primers that target the Y-chromosome DNA sequences in male plants.
• Understand how to extract DNA from their cannabis plants and prepare it for PCR analysis.
• Learn how to set up the PCR reaction on the Medicinal Genomics PCR machine.
• Analyze the results using gel electrophoresis, which allows for the separation and detection of DNA fragments based on their size.
• Interpret the results to determine the sex of their cannabis plants and make informed decisions about which plants to keep for their medicinal or recreational purposes.

By the end of this part of the master class, students will have gained knowledge of a cutting-edge technology that can identify the sex of cannabis plants at a young age, saving them weeks of waiting for the identification process to complete.

During this part of the cannabis tissue culture master class, students will learn about HpLVD (Hop latent viroid), a pathogen that can infect cannabis plants and cause significant damage to the crop.

• Students will learn about Agdia, a company that specializes in plant pathogen testing and offers a range of testing kits for different plant pathogens, including HpLVD.
• The class will cover the basics of HpLVD testing using Agdia kits, including sample collection, preparation, and testing protocols.
• Students will learn how to interpret the test results and identify infected plants, as well as how to implement appropriate management practices to control the spread of the pathogen.
• The class will also discuss the importance of pathogen testing in ensuring the health and quality of cannabis plants, particularly in the context of tissue culture propagation where pathogen-free plants are critical for success.

Synthetic Seeds Techniques: Cannabis is a plant with tantrums. It doesn't like it when it’s too hot or too cold. But, there’s a way you can do the cold storage of the plant by using synthetic seeds. Learn how to make them and use them to store Cannabis for a longer time.

During this part of the cannabis tissue culture master class, students will learn about long-term storage solutions for their cannabis plants, specifically the use of synthetic seeds.

• The focus will be on synthetic seeds, a technique used to store cannabis plant material at low temperatures.
• Students will learn how to make synthetic seeds using sodium alginate.
• They will also learn how to encapsulate cannabis explants in the alginate matrix and store them for long-term storage.
• Synthetic seeds provide a practical solution for the long-term storage of cannabis genetic material, as they are easy to transport and can be stored for many years.
• Students will also learn about other storage techniques, such as cryopreservation and slow growth storage, and their advantages and limitations.

This Master Class will allow you to get the knowledge and experience you need without all the costs that setting up the lab entails. Starting from getting access to all the info on Cannabis tissue culture, you will get the chance to connect with many Cannabis businesses and enthusiasts like you!

Further, do not forget to collect your Certificate to show the world your knowledge and expertise in the area.

Our last Master Class was a success, and our attendees say:

 “We got more than what we expected, and it has been a thrilling experience for us. No one gets such a level of hands-on at this price. I feel more confident now about tissue culture and using the technique to grow my business.”

This could also be you after joining our energizing “Tissue Culture Master Class: Cannabis.” 

Terms and Conditions: Plant Cell Technology, Inc reserves the right to cancel or change any class due to insufficient enrollment or other extenuating circumstances. Students enrolled in canceled classes will receive a credit towards the next master class of the same or lesser value. In the event of dates or instructor changes, all students will be notified. 

By attending the master class, you agree to the following statement:

" Understanding and assumption of risk. I understand there are inherent risks that cannot be eliminated in this activity. I have been advised of probable risks regarding the activities while at the Tissue Culture Master Class. I affirm that I have sufficient knowledge of the risks involved in this activity and understand that there are risks not specifically identified which are a part of the activity. I am aware of these risks and I agree to follow all safety instructions and to ask questions if I do not understand. I also acknowledge that, despite careful precautions, there are certain inherent risks of injury in this activity which each participant must assume if he or she desires to participate. Therefore, I assume and accept all risks of and full responsibility for bodily injury, death, loss of personal property and any expenses which may be suffered by me resulting from any identified or unidentified dangers and risks associated with my participation with my attendance to the Tissue Culture Master Class."